the Philippines. Some chemical properties of the samples were determined
using the Australia Standard methods To evaluate the phytotoxicity of coir
dust and coconut shell, a rapidbioassay method was used with lettuce
because the plant is a most sensitive species.
The moist sample of 50mL was weighed and placed in a petri dish to depth
about 9mm for the bioassay. The moisture of the sample was estimated
by its weight and bulk density. According to its moisture, water was
added to the petri dish to the water holding capacity of the sample. Ten
lettuce seeds were sown on the surface of the media in the petri dish. The
petri dish was covered with lid and incubated at room temperature
for 7 days. Finally, the root length was measured since the
roots were more sensitive to phytotoxins than shoots of lettuce. A known
nontoxic medium consisting of 0.5 L moist peat moss, 0.5 L sand, 4 g L1
dolomite, 0.75 gL1 KNO3, 1.0 gL1 single superphosphate, and
Sample pH (1:1.5) EC Cl
Fresh fine coir dust (0.25–1 mm) 6.11 0.80 450
Fresh coarse coir dust (1–2 mm) 5.89 0.60 300
Fresh coir dust (mixture) 5.90 0.68 364
Aged coir dust (unscreened) 5.89 0.51 300
Fresh coarse coconut shell (2–4 mm) 6.47 0.71 100
Fresh fine coconut shell (<2mm) 6.05 1.74 425 0.6 gL1 mixture of micronutrients as a bioassay control. There were two replications in the bioassay procedure. The phytotoxicity was expressed as the percentage of root length in the sample to the control. There was no fungal growth in petri dishes.To investigate the effects of incubation time, temperature and amendments on the phytotoxicity of fresh coir dust and shell, the samples of fresh coir and coconut were treated by (i) moistening with 500mLL1 water (moist), (ii) liming with 5 gL1 calcium carbonate(limed) and with 500mLL1 water, and (iii) plus 0.5 g L1 ferrous sulphate (limedþFe). The treated samples were kept in plastic bags and incubated in an incubator the dark and at different constant temperature systems. The samples were taken for bioassay every week for several weeks. After incubation, pH and EC of the incubated samples were determined in the suspension of sample and distilled water at the ratio of 1:1.5 .
using the Australia Standard methods To evaluate the phytotoxicity of coir
dust and coconut shell, a rapidbioassay method was used with lettuce
because the plant is a most sensitive species.
The moist sample of 50mL was weighed and placed in a petri dish to depth
about 9mm for the bioassay. The moisture of the sample was estimated
by its weight and bulk density. According to its moisture, water was
added to the petri dish to the water holding capacity of the sample. Ten
lettuce seeds were sown on the surface of the media in the petri dish. The
petri dish was covered with lid and incubated at room temperature
for 7 days. Finally, the root length was measured since the
roots were more sensitive to phytotoxins than shoots of lettuce. A known
nontoxic medium consisting of 0.5 L moist peat moss, 0.5 L sand, 4 g L1
dolomite, 0.75 gL1 KNO3, 1.0 gL1 single superphosphate, and
Sample pH (1:1.5) EC Cl
Fresh fine coir dust (0.25–1 mm) 6.11 0.80 450
Fresh coarse coir dust (1–2 mm) 5.89 0.60 300
Fresh coir dust (mixture) 5.90 0.68 364
Aged coir dust (unscreened) 5.89 0.51 300
Fresh coarse coconut shell (2–4 mm) 6.47 0.71 100
Fresh fine coconut shell (<2mm) 6.05 1.74 425 0.6 gL1 mixture of micronutrients as a bioassay control. There were two replications in the bioassay procedure. The phytotoxicity was expressed as the percentage of root length in the sample to the control. There was no fungal growth in petri dishes.To investigate the effects of incubation time, temperature and amendments on the phytotoxicity of fresh coir dust and shell, the samples of fresh coir and coconut were treated by (i) moistening with 500mLL1 water (moist), (ii) liming with 5 gL1 calcium carbonate(limed) and with 500mLL1 water, and (iii) plus 0.5 g L1 ferrous sulphate (limedþFe). The treated samples were kept in plastic bags and incubated in an incubator the dark and at different constant temperature systems. The samples were taken for bioassay every week for several weeks. After incubation, pH and EC of the incubated samples were determined in the suspension of sample and distilled water at the ratio of 1:1.5 .
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